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2020-1-1u2002·u2002Cellular and spatial sources of key cytokines in the bone marrow. ... bones were crushed in cell suspension medium (RPMI 1640 (Sigma) containing 2% fetal bovine serum) using a mortar and a pestle. Dissociated cells were filtered through a 40 μm filter, spun down at 1500 rpm for 5 min, then incubated with 5 ml ACK lysis buffer (Thermo Fisher ...
Get Price2021-10-5u2002·u2002Influenza A virus (IAV) and SARS-CoV-2 coinfection is a possible scenario during influenza season. Here, the authors show in a mouse model that IAV infection increases the risk of severe disease ...
Get Price2021-7-15u2002·u2002Cells were cultured in 5% CO 2 and maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U/mL penicillin, and 100 mg/mL ...
Get Price2021-12-1u2002·u2002HEK293T cells were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, and 10 × 10 −3 M sodium pyruvate. HEK293T-hACE2 cells were provided by M. Farzan and were cultured in DMEM with 10% FBS and 1% penicillin/streptomycin, 10 × 10 −3 M sodium pyruvate, and puromycin (2 ...
Get Price2021-9-8u2002·u2002Cytokine-based treatments for cancer have been hindered by both off-target toxicity and difficulty in achieving local delivery. To address these challenges, Hotz et al. developed a saline-formulated mixture of four mRNAs delivered by intratumoral injection.The mRNAs encoded interleukin-12 (IL-12) single chain, interferon-α, granulocyte-macrophage colony-stimulating …
Get PriceA full understanding of the relationship between surface properties, protein adsorption, and immune responses is lacking but is of great interest for the design of biomaterials with desired biological profiles. In this study, polyelectrolyte multilayer (PEM) coatings with gradient changes in surface wettability were developed to shed light on how this impacts protein adsorption and …
Get PricePreviously, preexisting humoral immunity had been largely analyzed using serum samples, not by studying memory B cells. Importantly, the fine specificity of memory B cells and plasma cells (responsible for serum IgG) can be different, as in the germinal center memory B cells differentiate from low-affinity precursors, whereas plasma cells ...
Get Price2018-3-28u2002·u2002Matrix-M-adjuvanted influenza vaccines induce influx of immune cells in the dLN with maintained composition of cellular subsets except for an increased monocyte population. a The total number of cells per dLN. b Contribution (%) of the indicated cellular subsets in the dLN was measured by flow cytometry at 4, 24, or 48 h after i.m. vaccination.
Get PriceExpatica is the international community's online home away from home. A must-read for English-speaking expatriates and internationals across Europe, Expatica provides a tailored local news service and essential information on living, working, and moving to your country of choice. With in-depth features, Expatica brings the international community closer together.
Get Price2021-12-18u2002·u2002Despite recent advancements, limitations in the treatment and control of hepatitis C virus (HCV) infection reprioritized the studies for invention of an efficient HCV vaccine to elicit strong neutralizing antibodies (NAbs) and cellular responses. Herein, we report molecular construction of a BacMam virus-based surface display for a subtype-1a HCV gpE2 (Bac-CMV …
Get Price2014-2-1u2002·u2002X-ray-irradiated DC (2 × 10 4) and the T cells (1 × 10 5) were seeded into wells of 96-well flat-bottom culture plates in RPMI 1640 10% fetal bovine serum (Invitrogen Life Technologies) for 3 days. Supernatants were removed and further analyzed for cytokine production (IL-10, TGF-β, IL-2 and IFN-γ) with ELISA.
Get Price2021-12-8u2002·u20021. Introduction. Since it was first reported in the 1980s, porcine reproductive and respiratory syndrome (PRRS) has been one of the most economically significant swine diseases worldwide [1,2].The causative agent, porcine reproductive and respiratory syndrome virus (PRRSV), is an enveloped virus with a single-stranded, positive-sense RNA genome of nearly 15 kb, …
Get Price2021-12-3u2002·u2002The coated plates were washed with PBS-T and incubated with 1% bovine serum albumin in PBS at 37 °C for 1 h. The serum was diluted from 1:20 for IgG antibodies, and the lung lavage fluids were diluted from 1:10 for IgA antibodies. The plates were washed with PBS-T 3 times, with a 150 μL volume for each well.
Get Price2021-12-23u2002·u2002Browse our listings to find jobs in Germany for expats, including jobs for English speakers or those in your native language.
Get Price2021-10-19u2002·u2002Briefly, bone marrow cells were flushed from the tibia and femurs of 8- to 12-week-old C57BL/6J mice. Cells were cultured in Dulbecco's Modified Eagle's Medium (Corning) supplemented with 10% fetal bovine serum (GIBCO), 1% GlutaMax (GIBCO), and 1% penicillin-streptomycin (GIBCO) and differentiated with 25 ng/ml mouse M-CSF (Peprotech) for 6 days.
Get Price2022-1-1u2002·u2002Chronic wounds represent a major health problem worldwide. Some of the available therapies based on recombinant proteins usually fail due to the hostile environment found at the wound bed. Aptamers appear as an attractive alternative to recombinant factors due in part to their stability, sensitivity, specificity and low-cost production. Herein, the Cell-SELEX technology was …
Get Price2021-12-2u2002·u2002Continuous antigen exposure drives CAR T cell exhaustion and promotes CD8+ T-to-NK-like T cell transition. Transcription factors ID3 and SOX4 are upregulated during CAR dysfunction and regulate genes associated with exhaustion, including NK receptors. Knocking out ID3 and SOX4 in CAR T cells slows dysfunction and improves anti-tumor immunity.
Get PriceThe process continues and glucagon is released as a result of cellular need for glucose, causing gluconeogenesis from stored sources in the liver and muscles and serum glucose levels continue to become elevated. Ketoacidosis patients present with elevated serum glucose (>300 mg/dL), ketones in the serum and urine, serum pH < 7.3, bicarb levels ...
Get Pricediabetes organ donation The EASD-ADA 2018 Type 2 Diabetes Consensus Statement. Mary Elizabeth Adams. Oct 12, 2018. type 2 diabetes treatment guidelines - EASD-ADA new ...
Get PriceThe process continues and glucagon is released as a result of cellular need for glucose, causing gluconeogenesis from stored sources in the liver and muscles and serum glucose levels continue to become elevated. Ketoacidosis patients present with elevated serum glucose (>300 mg/dL), ketones in the serum and urine, serum pH < 7.3, bicarb levels ...
Get Pricediabetes organ donation The EASD-ADA 2018 Type 2 Diabetes Consensus Statement. Mary Elizabeth Adams. Oct 12, 2018. type 2 diabetes treatment guidelines - EASD-ADA new ...
Get Price2020-1-1u2002·u2002Cellular and spatial sources of key cytokines in the bone marrow. ... bones were crushed in cell suspension medium (RPMI 1640 (Sigma) containing 2% fetal bovine serum) using a mortar and a pestle. Dissociated cells were filtered through a 40 μm filter, spun down at 1500 rpm for 5 min, then incubated with 5 ml ACK lysis buffer (Thermo Fisher ...
Get Price2021-10-5u2002·u2002Influenza A virus (IAV) and SARS-CoV-2 coinfection is a possible scenario during influenza season. Here, the authors show in a mouse model that IAV infection increases the risk of severe disease ...
Get Price2021-12-1u2002·u2002HEK293T cells were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, and 10 × 10 −3 M sodium pyruvate. HEK293T-hACE2 cells were provided by M. Farzan and were cultured in DMEM with 10% FBS and 1% penicillin/streptomycin, 10 × 10 −3 M sodium pyruvate, and puromycin (2 ...
Get Price2021-9-8u2002·u2002Cytokine-based treatments for cancer have been hindered by both off-target toxicity and difficulty in achieving local delivery. To address these challenges, Hotz et al. developed a saline-formulated mixture of four mRNAs delivered by intratumoral injection.The mRNAs encoded interleukin-12 (IL-12) single chain, interferon-α, granulocyte-macrophage colony-stimulating …
Get PriceA full understanding of the relationship between surface properties, protein adsorption, and immune responses is lacking but is of great interest for the design of biomaterials with desired biological profiles. In this study, polyelectrolyte multilayer (PEM) coatings with gradient changes in surface wettability were developed to shed light on how this impacts protein adsorption and …
Get PricePreviously, preexisting humoral immunity had been largely analyzed using serum samples, not by studying memory B cells. Importantly, the fine specificity of memory B cells and plasma cells (responsible for serum IgG) can be different, as in the germinal center memory B cells differentiate from low-affinity precursors, whereas plasma cells ...
Get Price2021-1-6u2002·u2002Tumor specimens were collected with patient consent and shipped immediately after surgery at 4°C for next day delivery in the tubes containing fresh media (RPMI-1640, 10% fetal bovine serum (FBS), 100 µg/mL penicillin and 100 µg/mL streptomycin).
Get Price2021-5-26u2002·u2002Cells were trypsinized (0.05% Trypsin-EDTA) and resuspended to a final cell density of 80,000 cells/mL in DMEM supplemented with 10% fetal bovine serum (FBS), seeded on the EM grids, and further ...
Get Price2021-12-3u2002·u2002Fluorescent probes that change their spectral properties upon binding to small biomolecules, ions, or changes in the membrane potential (V m) are invaluable tools to study cellular signaling pathways.Here, we introduce a novel technique for simultaneous recording of multiple probes at millisecond time resolution: frequency- and spectrally-tuned multiplexing …
Get Price2021-12-18u2002·u2002Despite recent advancements, limitations in the treatment and control of hepatitis C virus (HCV) infection reprioritized the studies for invention of an efficient HCV vaccine to elicit strong neutralizing antibodies (NAbs) and cellular responses. Herein, we report molecular construction of a BacMam virus-based surface display for a subtype-1a HCV gpE2 (Bac-CMV …
Get Price2020-1-1u2002·u2002Cellular and spatial sources of key cytokines in the bone marrow. ... bones were crushed in cell suspension medium (RPMI 1640 (Sigma) containing 2% fetal bovine serum) using a mortar and a pestle. Dissociated cells were filtered through a 40 μm filter, spun down at 1500 rpm for 5 min, then incubated with 5 ml ACK lysis buffer (Thermo Fisher ...
Get Price2021-10-5u2002·u2002Influenza A virus (IAV) and SARS-CoV-2 coinfection is a possible scenario during influenza season. Here, the authors show in a mouse model that IAV infection increases the risk of severe disease ...
Get Price2021-12-1u2002·u2002HEK293T cells were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, and 10 × 10 −3 M sodium pyruvate. HEK293T-hACE2 cells were provided by M. Farzan and were cultured in DMEM with 10% FBS and 1% penicillin/streptomycin, 10 × 10 −3 M sodium pyruvate, and puromycin (2 ...
Get Price2021-9-8u2002·u2002Cytokine-based treatments for cancer have been hindered by both off-target toxicity and difficulty in achieving local delivery. To address these challenges, Hotz et al. developed a saline-formulated mixture of four mRNAs delivered by intratumoral injection.The mRNAs encoded interleukin-12 (IL-12) single chain, interferon-α, granulocyte-macrophage colony-stimulating …
Get PriceA full understanding of the relationship between surface properties, protein adsorption, and immune responses is lacking but is of great interest for the design of biomaterials with desired biological profiles. In this study, polyelectrolyte multilayer (PEM) coatings with gradient changes in surface wettability were developed to shed light on how this impacts protein adsorption and immune ...
Get PricePreviously, preexisting humoral immunity had been largely analyzed using serum samples, not by studying memory B cells. Importantly, the fine specificity of memory B cells and plasma cells (responsible for serum IgG) can be different, as in the germinal center memory B cells differentiate from low-affinity precursors, whereas plasma cells ...
Get Price2021-1-6u2002·u2002Tumor specimens were collected with patient consent and shipped immediately after surgery at 4°C for next day delivery in the tubes containing fresh media (RPMI-1640, 10% fetal bovine serum (FBS), 100 µg/mL penicillin and 100 µg/mL streptomycin).
Get Price2021-5-26u2002·u2002Cells were trypsinized (0.05% Trypsin-EDTA) and resuspended to a final cell density of 80,000 cells/mL in DMEM supplemented with 10% fetal bovine serum (FBS), seeded on the EM grids, and further ...
Get Price2021-12-3u2002·u2002Fluorescent probes that change their spectral properties upon binding to small biomolecules, ions, or changes in the membrane potential (V m) are invaluable tools to study cellular signaling pathways.Here, we introduce a novel technique for simultaneous recording of multiple probes at millisecond time resolution: frequency- and spectrally-tuned multiplexing (FAST M).
Get Price2021-12-18u2002·u2002Despite recent advancements, limitations in the treatment and control of hepatitis C virus (HCV) infection reprioritized the studies for invention of an efficient HCV vaccine to elicit strong neutralizing antibodies (NAbs) and cellular responses. Herein, we report molecular construction of a BacMam virus-based surface display for a subtype-1a HCV gpE2 (Bac-CMV-E2 …
Get PriceCellular and spatial sources of key cytokines in the bone marrow. ... bones were crushed in cell suspension medium (RPMI 1640 (Sigma) containing 2% fetal bovine serum) using a mortar and a pestle. Dissociated cells were filtered through a 40 μm filter, spun down at 1500 rpm for 5 min, then incubated with 5 ml ACK lysis buffer (Thermo Fisher ...
Get PriceInfluenza A virus (IAV) and SARS-CoV-2 coinfection is a possible scenario during influenza season. Here, the authors show in a mouse model that IAV infection increases the risk of severe disease ...
Get PriceHEK293T cells were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, and 10 × 10 −3 M sodium pyruvate. HEK293T-hACE2 cells were provided by M. Farzan and were cultured in DMEM with 10% FBS and 1% penicillin/streptomycin, 10 × 10 −3 M sodium pyruvate, and puromycin (2 ...
Get PriceCytokine-based treatments for cancer have been hindered by both off-target toxicity and difficulty in achieving local delivery. To address these challenges, Hotz et al. developed a saline-formulated mixture of four mRNAs delivered by intratumoral injection.The mRNAs encoded interleukin-12 (IL-12) single chain, interferon-α, granulocyte-macrophage colony-stimulating factor, and IL-15 sushi.
Get PriceA full understanding of the relationship between surface properties, protein adsorption, and immune responses is lacking but is of great interest for the design of biomaterials with desired biological profiles. In this study, polyelectrolyte multilayer (PEM) coatings with gradient changes in surface wettability were developed to shed light on how this impacts protein adsorption and immune ...
Get PricePreviously, preexisting humoral immunity had been largely analyzed using serum samples, not by studying memory B cells. Importantly, the fine specificity of memory B cells and plasma cells (responsible for serum IgG) can be different, as in the germinal center memory B cells differentiate from low-affinity precursors, whereas plasma cells ...
Get PriceTumor specimens were collected with patient consent and shipped immediately after surgery at 4°C for next day delivery in the tubes containing fresh media (RPMI-1640, 10% fetal bovine serum (FBS), 100 µg/mL penicillin and 100 µg/mL streptomycin).
Get PriceCells were trypsinized (0.05% Trypsin-EDTA) and resuspended to a final cell density of 80,000 cells/mL in DMEM supplemented with 10% fetal bovine serum (FBS), seeded on the EM grids, and further ...
Get PriceFluorescent probes that change their spectral properties upon binding to small biomolecules, ions, or changes in the membrane potential (V m) are invaluable tools to study cellular signaling pathways.Here, we introduce a novel technique for simultaneous recording of multiple probes at millisecond time resolution: frequency- and spectrally-tuned multiplexing (FAST M).
Get PriceDespite recent advancements, limitations in the treatment and control of hepatitis C virus (HCV) infection reprioritized the studies for invention of an efficient HCV vaccine to elicit strong neutralizing antibodies (NAbs) and cellular responses. Herein, we report molecular construction of a BacMam virus-based surface display for a subtype-1a HCV gpE2 (Bac-CMV-E2-gp64; Bac) that both expressed ...
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