Combined single-cell and spatial transcriptomics reveals ...
2020-1-1u2002·u2002Cellular and spatial sources of key cytokines in the bone marrow. ... bones were crushed in cell suspension medium (RPMI 1640 (Sigma) containing 2% fetal bovine serum) using a mortar and a pestle. Dissociated cells were filtered through a 40 μm filter, spun down at 1500 rpm for 5 min, then incubated with 5 ml ACK lysis buffer (Thermo Fisher ...
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